Coding

Part:BBa_K3317014:Design

Designed by: Julieta Monserrat Melendez Calvo and Luis Mario Zepeda Guzman   Group: iGEM19_UANL   (2019-10-16)


S. enterica pduU modified protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sequence optimized to e. coli BL21, the ecoRI, XbaI, SpeI and PstI sites were avoided, a signal peptide () was added at its C terminal end together with a flexible G4S linker


Source

adapted to e.coli from s. enterica LT2 genome (NC_003197.1),

References